Resumen (es):
El presente trabajo colectó, aisló, identificó y caracterizó el potencial antimicrobiano de dos especies filamentosas de cianobacterias de la Amazonia colombiana. Se utilizaron técnicas microbianas de rayado en medio sólido y dilución para obtener las cepas con una sola especie. La identificación se realizó por caracteres morfológicos y secuenciado de la sección conservada Cya de gen 16s. Se identificaron por reacción en cadena de la polimerasa (PCR) la presencia de genes que secuencian policétidos sintasas (PKS) y péptidos no ribosomales sintetasas (NRPS) asociados a rutas productoras de metabolitos secundarios. Se evaluó la actividad antimicrobiana de extractos polares y no polares con una batería Gram positiva, dos Gram negativas y una cepa fúngica. Se identificó la cepa 5 como Limnothrix vacuolifera (Skuja) Komárek y la cepa 9 como Lymnothix planktonica (Wołoszyńska) Meffert. En las dos cepas se encontraron PKS y NRPS. Finalmente, los extractos polares inhibieron el crecimiento de Enterococcus faecalis, Escherichia coli y Klebsiella pneumoniae y una cepa Penicillium sp. Los extractos no polares no mostraron actividad antimicrobiana. Es el primer registro de estas para la Amazonia colombiana y el primer registro de actividad antibiótica de cianobacterias continentales colombianas.
Resumen (en):
In recent years Cyanobacteria have become an important source of secondary metabolites. The present work collected, isolated, identified, and characterized the antimicrobial potential of two filamentous species of cyanobacteria from the Colombian Amazon. Microbial techniques streaking in solid medium and dilution in liquid medium were used to obtain strains with a single species. Identification was performed by morphological characters and sequencing the conserved Cya section of the 16s gene. The presence of genes sequencing polyketide synthases (PKS) and non-ribosomal peptide synthetases (NRPS) associated with secondary metabolite-producing pathways were identified by PCR. The antimicrobial activity of polar and non-polar extracts was evaluated with one Gram-positive and two Gram-negative bacteria and a fungal strain. Strain 5 was identified as Limnothrix vacuolifera (Skuja) Komárek and strain 9 as Lymnothix planktonic (Wołoszyńska) Meffert. In both strains PKS and NRPS were found. Finally polar extracts inhibited the growth of Enterococcus faecalis, Escherichia coli and Klebsiella pneumoniae and one strain Penicillium sp. Non-polar extracts didn’t show antimicrobial activity. This is the first record of these for the Colombian Amazon, and the first record of antibiotic activity of Colombian continental cyanobacteria.
Palabras clave:
Amazonia colombiana, antibiótico, antifúngicos, cianobacteria, metabolitos secundarios (es)
Colombian Amazon, antibiotic, antifungal, cyanobacteria, secondary metabolites (en)
Colombian Amazon, antibiotic, antifungal, cyanobacteria, secondary metabolites (en)
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Cómo citar
Andrade Silva, A., Montenegro Ruiz, L. C., Duque Escobar, S. R., & Cano Arango, J. E. (2023). Primer registro de Limnothrix vacuolifera y Limnothrix planktonica (Cyanobacteria) en la Amazonia colombiana y su actividad antimicrobiana. Biota Colombiana, 25, e1171. https://doi.org/10.21068/2539200X.1171
Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-SinDerivadas 4.0.
Derechos de autor 2023 Instituto de Investigación de Recursos Biológicos Alexander Von Humboldt
